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2 edition of In vitro analysis of the microenvironmental regulation of murine B lymphocyte development. found in the catalog.

In vitro analysis of the microenvironmental regulation of murine B lymphocyte development.

Robert Joseph Ray

In vitro analysis of the microenvironmental regulation of murine B lymphocyte development.

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  • 27 Currently reading

Published by National Library of Canada = Bibliothèque nationale du Canada in Ottawa .
Written in English


Edition Notes

SeriesCanadian theses = Thèses canadiennes
The Physical Object
Pagination206 leaves.
Number of Pages206
ID Numbers
Open LibraryOL18494591M
ISBN 100612277143

Jan 01,  · Although we have gained much information about lead-induced organ damage, the effect of blood lead level on T cell subgroup is yet to be determined. To assess the effect of blood lead level on T-lymphocyte Subpopulation Expression of children, and the association of T-lymphocyte Subpopulation Expression with threshold limit value of blood lead amstrad.fun aim of study is evaluating the Author: Lin Li, Qing He Meng, Shi Guang Li, Qing Hao Kong. Cytochalasin B was added to the cultures at 44 hours post initiation as described by Fenech and Morley (). Cytochalasin B prevents the cells from completing cytokinesis resulting in the formation of multinucleated cells. Final concentration of cytochalasin B in the cell cultures was 3 mg/ml. of analysis of variance and Duncan's new multiple range test indicated that vitro for cell-mediated tissue damage in vivo (1). When cytotoxicity is in-duced by stimulation of lymphocytes, to suppression of the mixed lymphocyte-erythrocyte aggregation normally causedCited by: The genetic, endocrine, and metabolic mechanisms underlying female reproduction are numerous and sophisticated, displaying complex functional evolution throughout a woman’s lifetime. This vital course may be systematized in three subsequent stages: prenatal development of ovaries and germ cells up until in utero arrest of follicular growth and the ensuing interim suspension of gonadal Cited by: 4.


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In vitro analysis of the microenvironmental regulation of murine B lymphocyte development. by Robert Joseph Ray Download PDF EPUB FB2

Abstract. Lymphocyte development is a dynamic process in which committed lymphoid precursors progress through a series of defined maturational stages before generating B- and T-cells that express immunoglobulin and the T-cell receptor (TCR), respectively (1–4).This process is regulated by a variety of increasingly well-defined extracellular signals that influence the growth and Cited by: 5.

Mar 07,  · Adiponectin stimulates the production of a B-lymphocyte derived peptide which indirectly downregulates the affinity of adhesion molecules on T-lymphocytes via induction of endothelial sphingosinephosphate (Fig.

The effects of adiponectin have. In vitro development of B lymphocytes from long-term cultured precursor cells. Abstract. An in vitro system for the study of B-lymphocyte differentiation from precursor elements has been established. Spooncer E, Toksoz D, Lajtha LG. The role of cells and their products in the regulation of in vitro stem cell proliferation and Cited by: Oct 01,  · Regulation of the growth and functions of cloned murine large granular lymphocyte lines by resident macrophages.

The effect of natural killer cells on the development of syngeneic hematopoietic progenitors. J Immunol. In vitro analysis of the microenvironmental regulation of murine B lymphocyte development. book Dec; (6)– The development and functional activities of B lymphocytes critically depend on their migratory capacity.

Both in vitro and in vivo assays can be used to assess the migratory ability of B cells. ELSEVIER Journal of Immunological Methods () JOURNAL OF IMMUNOLOGICAL METHODS Transient transfection of murine B lymphocyte blasts as a method for examining gene regulation in primary B cells Steven B.

McMahon, Amanda Norvell, Karen J. Levine, John G. Monroe Department of Pathology and Laboratory Medicine, Room A, Curie Blvd., School of Medicine, Cited by: COMPUTER ANALYSIS OF DEFINED POPULATIONS OF LYMPHOCYTES IRRADIATED VITRO IN III. Evaluation of Human T and B Peripheral Blood Origin* George B.

Olson, Ph.D.,t Robert E. Anderson, M.D.,+~ and Peter H. Bartels, Ph.D. Cells of Abstract The radiosensiti~ities of lymphocytes of peripheral blood-origin obtained from two healthy 45)'ear old male donors were studied st~'mtiltaneously at Cited by: Apr 11,  · A murine local lymph node assay for the identification of contact allergens has been developed.

Contact sensitizing activity is measured as a function of lymphocyte proliferation in the draining lymph node following repeated application of the test agent to the dorsum of the ear.

Regulation of B Lymphocyte and Macrophage Development by Graded Expression of PU.1 Article (PDF Available) in Science () · June with Reads How we measure 'reads'.

At no time in recent memory has it been so difficult to offer succint generalizations about the biology of the mast cell, or its role in health and disease. This situation is something of a paradox, Cited by: MURINE B LYMPHOCYTE GROWTH REGULATION A study of host defence factors and their mimicry by Mycoplasma arginini supporting the development of Ig-secreting plaque-forming cells (PFC) in B cell blast supporting the growth of LPS-preactivated murine spleen B cells was found as a highly.

Apr 12,  · Hematopoiesis, or the process of blood formation, has been extensively studied at both basic and clinical levels. Human diseases such as thalassemia, immunodeficiency, and leukemia represent defects in this process. Approaches to treat these disorders have required a basic understanding of the biology of blood cells.

For instance, hemapoietic stem cell replacement or bone 3/5(1). Effects of b-carotene and astaxanthin on murine lymphocyte functions and cell surface marker expression in in vitro culture system. Nutr CancerJyonouchi H, Voss RM, and Good RA: Zn depletion modifies CD5 expression by 7OZ/3 murine pre-B leukemia cell line.

Proc Soci Exp Biol MedClassification of the various cell types has been based so far on their phenotypic properties (e.g., membrane antigen markers, cell receptors, production and secretion of immunoglobulins, etc.). Genotypically, T and B cells may represent either separate, independent cell lines, or different stages of development within the same cell amstrad.fun by: 5.

Lung Lymphocyte Elimination by Apoptosis in the Murine Response to olar lining fluid also inhibits lymphocyte proliferation in vitro, because of both lipid and protein components (9, 10). Thus, Analysis of lymphocyte DNA fragmentation in isolated nuclei by gel electrophoresis.

Cadiele Oliana Reichert, Joel da Cunha, Débora Levy, Luciana Morganti Ferreira Maselli, Sérgio Paulo Bydlowski and Celso Spada (July 12th ). Regulatory T Lymphocytes (Treg): Modulation and Clinical Application, Lymphocyte Updates - Cancer, Autoimmunity and Infection, Gheorghita Isvoranu, IntechOpen, DOI: /intechopenCited by: 2.

Start studying Lymphocytes and Plasma Cells (Chap 16). Learn vocabulary, terms, and more with flashcards, games, and other study tools. Consider a single murine T lymphocyte circulating through the bloodstream and at homeostasis with its surrounding environment.

Due to exposure of the organism to an external pathogen that infiltrates the blood, this lymphocyte receives signals that trigger the need to synthesize exactly 15 copies of a protein required for mediating an inflammatory response. At MuriGenics we perform a variety of in vitro toxicity screens and assays for your drug candidates.

Performing in vitro cytotoxicity assays prior to animal testing may be a cheaper alternative for initial drug candidate screens. Lafreniere, R., Rosenstein, M., & Rosenberg, S. In vitro expansion of lymphokine-activated killer cells leads to increased numbers of cells which can mediate the reduction of murine hepatic and pulmonary metastases when adoptively transferred with recombinant interleukinAuthor: R.

Lafreniere, M. Rosenstein, S. Rosenberg. Jan 15,  · Biology of the B Lymphocyte. B lymphocytes acquired their name from early experiments in chickens. The synthesis of antibody was shown to require the presence of an organ called the bursa of Fabricius (an outpouching of the cloacal epithelium).

Surgical removal of the bursa early in life prevented antibody synthesis. An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro Gareth Pryce, Washington Santos and David Male * Department of Neuropathology, Institute of Psychiatry, De Crespigny Park, London SE5 8AF, UK and development of immune responses in the brain.

The level of cell migration into brain is normally very low in. Epigenetic regulation of B cell development and immunoglobulin gene recombination. The main function of these cells is the production of antibodies. They can also act as APC for T cells. Many epigenetic changes have been described during B cell development, differentiation, and effector amstrad.fun: Cristian Doñas, Alejandra Loyola, Mario Rosemblatt.

Original Article Silencing the expression of Cbl-b enhances the immune activation of T lymphocytes against RM-1 prostate cancer cells in vitro Shu-Kui Zhou, Wei-Hua Chen, Zheng-Duo Shi, Shun-Ping Wang, Liang Li, Xiao-Fei Wen. Flow cytometric analysis of single cell suspensions of small-intestinal lymphocytes should yield a discrete population of cells that have similar forward and side scatter characteristics as splenocytes (Figures 1A and 1B).The lymphocytes may begin to die if the tissue is not maintained at 4 °C during the initial stages of the isolation, resulting in the lymphocyte population having a lower.

Dec 01,  · 3. Cytokine Expression in Nonclassical MPN: Systemic Mastocytosis. SM is MPN characterized by an abnormal accumulation of mast cells in the BM and other organs [].In a substantial subset of patients, SM is accompanied by increased release of various mediators from mast cells and consecutive clinical symptoms [–].The majority of SM patients harbor the somatic KIT point.

Furthermore, a post-hoc analysis of gene expression in ER-positive breast cancer patients showed that treatment with the AI letrozole increased the infiltration of B cell and T helper lymphocyte subsets at early and late time points following treatment amstrad.fun by: lymphocyte proliferative responses observed in healthy, immunologically competent individuals at large.

The reference ranges provided will be helpful in ascertaining the magnitude of the normal response. Lymphocyte proliferation to mitogens is known to be affected by concomitant use of steroids, immunosuppressive agents, including.

After birth, the development of hematopoietic cells occurs in the bone marrow. Hematopoietic differentiation is finely tuned by cell-intrinsic mechanisms and lineage-specific transcription factors.

However, it is now clear that the bone marrow microenvironment plays an essential role in the maintenance of hematopoietic stem cells (HSC) and their differentiation into more mature lineages Cited by: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences.

The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological Cited by: 5.

Voss RM, and Good RA: Up-regulation and down-regulation of cell surface and mRNA expression of CD5 antigen by various humoral factors on murine 70Z/3 pre-B cell leukemia cell line. IL-4 down-regulates CD5 antigen expression.

Cell Immunol, Estimate proliferation in cell-tracking dye studies Davide Rambaldi October 29, Abstract Background In cells proliferation tracking experiments, cells are stained with a tracking dye before culture.

During cell division, the dye will be divided between daughter cells so that each daughter cell brings about a. Nov 06,  · Physiological interactions between the nervous and immune systems with components of the local microenvironment are needed to maintain homeostasis throughout the body.

Dynamic regulation of bone remodeling, hematopoietic stem cells, and their evolving niches via neurotransmitter signaling are part of the host defense and repair mechanisms. This crosstalk links activated leukocytes, neuronal Cited by: Our lab is interested in the impact of aging on the cellular and molecular regulation of B-lymphocyte development and B-cell homeostasis.

B-cells form and reach an immature, but responsive stage, in the hemopoietic tisssues of fetal liver and postnatal bone marrow.

This article has no abstract; the first words appear below. Only recently have concepts of delayed hypersensitivity evolved from the dark mire of an ill defined, firm, red bump in the skin to Cited by: 5.

Intrinsic versus microenvironmental regulation of lymphatic endothelial cell phenotype and function TANJA VEIKKOLA, MARJA LOHELA, KRISTIAN IKENBERG,* TAIJA MA¨KINEN, THOMAS KORFF,* ANNE SAARISTO, TATANIA PETROVA, MICHAEL JELTSCH, HELLMUT G.

Cited by: Editorial from The New England Journal of Medicine — Microenvironmental Protection in Diffuse Large-B-Cell Lymphoma studies and expression profiling for use in genetic analysis, molecular Cited by: 6. Dec 06,  · Toni Lindl, Birgit Lewandowski, Sonya Schreyögg and Andrea Stäudte According to the National Institutes of Health guidance document on using in vitro data to estimate in vivo starting doses for acute toxicity, the performance of the electrical current exclusion method (ECE) was studied for its suitability as an in vitro cytotoxicity test.

In a comparative. Oct 29,  · The receptor IL-7R is expressed on immature B-cell progenitors, and IL-7 appears to be critical in B-cell development. Unlike IL-2, IL-7 selectively expands CD8 T cells over Tregs. IL-7 agonists are currently in clinical development and appear to be well tolerated and promote antitumor immune responses.

Cited by: Human bone marrow derived mesenchymal stem cells (BM-MSCs) resides in their niches in close proximity to hematopoietic stem cells (HSCs). These naïve MSCs have tremendous potential in regenerative therapeutics, and may also be exploited by cancer and infectious disease agents.

Hence, it is important to study the physiological and pathological roles of naïve MSC. However, our knowledge Cited by: 7. Neural Crest and the Development of the Enteric Nervous System Book New role for the (pro)renin receptor in T-cell development Academic Article Oct-2 Gene Disruption Eliminates the Peritoneal B-1 Lymphocyte Lineage and Attenuates B-2 Cell Maturation and Function Academic Article.Oct 01,  · We have examined the occurrence and distribution of HP1α and HP1β under in vivo, ex vivo and in vitro conditions.

Consistent with a non-essential role in heterochromatin maintenance, both proteins are diminished or undetectable in several types of differentiated cells and are universally downregulated during erythropoiesis.

Variant-specific patterns are observed in almost all human and Cited by: In vitro evaluation, biodistribution and scintigraphic imaging in mice of radiolabeled anthrax toxins Ekaterina Dadachovaa,b,⁎, Johanna Riverab, Ekaterina Revskayaa, Antonio Nakouzib, Sean M.

Cahillc, Michael Blumensteind, Hui Xiaoe, Dmitry Rykunovc, Arturo Casadevallb,f aDepartment of Nuclear Medicine, Albert Einstein College of Medicine, Bronx, NYUSA.